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Angela Asirvatham



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Creatine is an important component of the high-energy phosphate transfer to regulate cellular levels of ATP. The functions of creatine supplementation are well studied in the muscular and skeletal system, however the impact of creatine in the nervous system, specifically Schwann cells, is relatively unknown. Schwann cell growth in vitro is facilitated by heregulin, a neuron secreted growth factor, and forskolin, a pharmacological agent that activates cAMP. In peripheral nervous system injuries, recovery time is based around Schwann cell’s ability to proliferate and differentiate, and it is unknown what role creatine plays in this process. It was hypothesized that increasing creatine concentrations in combination with growth factors would augment Schwann cell growth. Immortalized Schwann cell line S16 cells were treated with N2 (control), heregulin (12.5ng/mL), forskolin (2µM) and heregulin plus forskolin for 24 hours. To determine the optimal dose of creatine for the Schwann cells, doses of 200nM creatine, 2µM creatine, and 20µM creatine were added to each of the previously stated treatments, and was repeated for 1-, 6-, and 12-hour timepoints. At the 1- and 6-hour time points 200nM and 2µM showed the greatest proliferation amongst the three doses and, the cells were most influenced by the presence of heregulin (108.9±5.45), (113.3±20.1). A synergistic effect was noted between heregulin and forskolin at both time points (104.86±2.9), (107.76±20.88). At the 12- and 24-hour time points the optimal creatine dosage for proliferation was 2µM and, the cells were most influenced by the presence of forskolin (108.38±7.6), (110.52±1.60). These time points showed a synergistic effect as well. This suggests that at shorter timepoints, creatine stimulates a different pathway than at longer timepoints. In summary, these findings suggest that creatine stimulate proliferation through different pathways depending on concentration and timepoint.

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Schwann cells, cell signaling, cAMP, heregulin, dose response


Biological Phenomena, Cell Phenomena, and Immunity | Life Sciences | Medical Cell Biology

The Effect of Creatine on Immortalized Schwann Cell Proliferation