Faculty Advisor(s)

Angela Asirvatham

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Abstract

Bisphenol A is a harmful compound common in plastics that causes negative health and environmental effects. Due to these effects, new guidelines have been made to limit exposure to BPA. However, modified versions of these compounds, including Bisphenol-F, are being used as a replacement. Exposure to BPF, which is comparable in structure to BPA, has similar effects as BPA on many organ systems with some being unknown. As there is not much known about BPF, especially in the peripheral nervous system, the aim of this research is to analyze proliferation rates in BPF-treated Schwann cells. It was hypothesized that with increased concentrations of BPF, there will be an increase in cell proliferation of RT4 Schwannoma cancer cells in comparison to cells without BPF. To investigate this hypothesis, RT4 cells were treated with BPF and assayed for cell division using an EdU cell proliferation assay. The EdU positive ration and cell density of each group under different concentrations was calculated after completing the EdU incorporation assay. Results indicate that higher dosages of BPF beyond 1x106 M (28.99% ± 0.107) did not elicit an increase in proliferation, although a concentration of 10-4 M (34.08% ± 0.123) slightly increased growth. The BPF treatment at a concentration of 10-7 M had the greatest percent change (43.6% ± 0.107). Based on these results, it was concluded that low concentrations (1x10-7 M) of BPF were sufficient to increase cell proliferation which has implications in the health of the peripheral nervous system.

Publication Date

2024

Document Type

Poster

Department

Biology

Keywords

BPA, BPF, Schwannoma cells, cell proliferation

Disciplines

Biology | Cell Biology | Life Sciences

The Effects of BPF on RT4 Schwannoma Cancer Cells

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