Faculty Advisor(s)

Angela Asirvatham

Download

Download Poster (156 KB)

Abstract

Schwann cells have been found to play a key role in inflammation and repair following nerve injury. During nerve injury, the myelin sheath becomes damaged, stimulating Schwann cells to secrete cytokines and initiate an inflammatory response. They begin the process of repair by stimulating macrophages to make pro-inflammatory cytokines, such as Tumor Necrosis Factor-Alpha (TNF-a), and anti-inflammatory cytokines, such as Interleukin 10 (IL-10) which promotes healing and injury. Although it is clear Schwann cells play a role in nerve repair, there is still yet to be known regarding the exact mechanisms by which they do so. In vitro, Lipopolysaccharide (LPS), can induce systematic inflammation and stimulate the production of inflammatory mediators, like tumor necrosis factor alpha (TNF-α) and Interleukin 10 (IL-10). Reports on LPS-stimulated microglial cells treated with forskolin at various time points revealed an increase in IL-10 mRNA levels. A similar study conducted on LPS-treated RT4-Schwann cell lines that were stimulated with forskolin for 3 hours resulted in the secretion of TNF- α. To further explore the effects of forskolin in LPS-stimulated Schwann cells, the role of IL-10 secretion was examined. It was hypothesized that IL-10 secretion will be increased in LPS-treated Schwann cells and IL-10 secretion will be higher in LPS-treated Schwann cells that are stimulated with forskolin. Cells from the RT4-D6P2T Schwann cell line were treated with 0.1,1, or 10 μg/mL of LPS, or 1, 2, 3 μM forskolin (F), or combinations of LPS and F treatments for 3 and 24 hours in N2 media in a 24 well plate. Using the ELISA, IL-10 secretion, was higher in LPS + F treated cells than in LPS-stimulated or F treated cells only. IL-10 was increased at 1 μg/mL of LPS with all treatments of F at 24 hours (404+/- 231.3%, 586+/-226.2%, 474+/-299.1%). Surprisingly, IL-10 was higher in LPS-stimulated cells at 3 hours with 10 μg/mL of LPS + 3 μM F(462+/-60.35%) than at 24 hours (66+/-66.3%). In summary, it appears as though all the combination treatments of LPS and F factors, may act in unity to upregulate IL-10, while being dependent on time. These findings suggest that, during nerve injury, the cAMP pathway may have the ability to counteract inflammation by secreting IL-10 in LPS-stimulated Schwann cells is dependent on the concentration of and duration. A better understanding of the balance between the pro and anti-inflammatory cytokines during nerve injury may provide more information.

Publication Date

2025

Document Type

Poster

Department

Biology

Disciplines

Biology | Life Sciences

The Involvement of cAMP in the Secretion of IL-10

Included in

Biology Commons

Share

COinS